RT Journal Article
SR Electronic
T1 Evaluation of Disinfectants on Military NATO and DECON Litters
JF American Society for Clinical Laboratory Science
JO Clin Lab Sci
FD American Society of Chemistry and Laboratory Science
SP 102
OP 106
DO 10.29074/ascls.21.2.102
VO 21
IS 2
A1 Hensley, Donna M.
YR 2008
UL http://hwmaint.clsjournal.ascls.org/content/21/2/102.abstract
AB OBJECTIVE: This study evaluated the effectiveness of five disinfectants: A33, 10% bleach, 1% bleach, SPOROX, and 3% H2O2, on military NATO and DECON litters.DESIGN: Suspensions of Acinetobacter baumannii, Staphylococcus aureus, and spore-enhanced Bacillus subtilis, with five percent albumin, were inoculated onto litters and dried overnight. The litters were saturated with disinfectant solutions and sampled after 10 minutes. The Log10 reduction in the number of bacteria recovered was calculated.SETTING: 59th Medical Wing, 59th Clinical Research Division, Lackland AFB TX.MAIN OUTCOME MEASURES: A reduction of ≥3 Log10 in the number of bacteria recovered from the test squares compared to the control squares was considered effective disinfection.RESULTS: On the NATO litter 10% bleach and SPOROX were effective against vegetative cells. On the DECON litter A33, 10% bleach, and SPOROX were effective against vegetative cells. After the 10 minute exposure none of the disinfectants evaluated were effective against spore-enhanced B. subtilis.CONCLUSION: When contaminated with vegetative cells military NATO and DECON litters can be effectively dis-infected with a 10 minute exposure to some disinfectants. Further research is needed to find an effective disinfectant for spore contamination.ABBREVIATIONS: AFB = Air Force Base; ATCC = American Type Culture Collection; BAMC = Brooke Army Medical Center; BSA = bovine serum albumin; CFU = colony forming units; D/E = Dey/Engley; DECON = decontamination; H2O2 = hydrogen peroxide; NAM = nutrient agar with manganese sulfate (50 μg/mL); NATO = North Atlantic Treaty Organization; QAC = quaternary ammonium compound; SBA = Trypticase soy agar with five percent sheep's blood.