RT Journal Article SR Electronic T1 Methicillin-resistant Staphylococcus aureus Fomite Survival JF American Society for Clinical Laboratory Science JO Clin Lab Sci FD American Society of Chemistry and Laboratory Science SP 34 OP 38 DO 10.29074/ascls.22.1.34 VO 22 IS 1 A1 Christa Williams A1 Diane L Davis YR 2009 UL http://hwmaint.clsjournal.ascls.org/content/22/1/34.abstract AB OBJECTIVE: To assess survival of methicillin-resistant Staphylococcus aureus (MRSA) on fomites encountered by health students.DESIGN: Three suspensions of MRSA were made to mimic lab splashes: a 0.5 McFarland trypticase soy broth, whole blood with 50 colony forming units/mL and body fluid/serum with 2,000 colony forming units/mL. These were seeded onto three environmental surfaces (glass, vinyl floor tile, and countertop) and wet swabbed for 60 days. High touch areas of student stethoscopes were also wet swabbed. MRSA selective CHROMagar® was used to identify organism survival.SETTING: Salisbury University, Salisbury MDPARTICIPANTS: Salisbury University nursing and respiratory therapy students who volunteered to have their stethoscopes swabbed anonymously.MAIN OUTCOME MEASURE: Detection of pink colonies on MRSA-selective CHROMagar®.RESULTS: MRSA in 0.5 McFarland broth lived for >60 days on all surfaces. MRSA in blood was undetectable on any surface, and MRSA in serum survived 41 days on glass, 45 days on tile, and >60 days on countertop. Five of thirty-three stethoscopes (15%) tested were positive for MRSA.CONCLUSIONS: Previous studies showed fomite survival of MRSA for about two weeks using contact plate sampling and MRSA on 7.4% of stethoscopes. We showed longer MRSA survival times by wet swab sampling and a higher stethoscope contamination rate. As expected, higher organism loads survived longer.ABBREVIATIONS: ATCC = American Type Colony Collection; CFUs = colony forming units; MRSA = methicillin-resistant Staphylococcus aureus; MSSA = methicillin-sensitive Staphylococcus aureus; TS = trypticase soy.