PT  - JOURNAL ARTICLE
AU  - Donna M. Hensley
AU  - David L. McGlasson
AU  - Kevin J Krauland
TI  - <em>Acinetobacter baumannii</em> and MRSA Contamination on Reusable Phlebotomy Tourniquets
AID  - 10.29074/ascls.23.3.151
DP  - 2010 Jul 01
TA  - American Society for Clinical Laboratory Science
PG  - 151--156
VI  - 23
IP  - 3
4099  - http://hwmaint.clsjournal.ascls.org/content/23/3/151.short
4100  - http://hwmaint.clsjournal.ascls.org/content/23/3/151.full
SO  - Clin Lab Sci2010 Jul 01; 23
AB  - OBJECTIVE: A study was undertaken to determine the incidence of Acinetobacter baumannii and methicillin resistant Staphylococcus aureus (MRSA) contamination on reusable phlebotomy tourniquets at Wilford Hall Medical Center, Lackland AFB, TX.DESIGN: Reusable tourniquets (n=200) were collected after being used for one day in the outpatient blood collection center (n=100) or during morning blood collection rounds on inpatient wards (n=100). Tourniquets were cultured and growth was screened for A. baumannii and S. aureus. A. baumannii isolates were identified using colonial morphology, oxidase, and GNI+ card on Vitek Legacy. S. aureus isolates were identified and screened for MRSA using colonial morphology, catalase, Staphaurex, and Oxacillin screening agar.RESULTS: Each outpatient tourniquet was used on an average of 33 patients and each inpatient tourniquet was used on an average of 11 patients. The overall contamination rate was 9% (18/200). A. baumannii was isolated from 11% (11/100) of the outpatient tourniquets and 3% (3/100) of the inpatient tourniquets. Methicillin-susceptible S. aureus was isolated from 2% (2/100) of the outpatient tourniquets and 3% (3/100) of the inpatient tourniquets. No MRSA was isolated. One outpatient tourniquet had both A. baumannii and methicillin-susceptible S. aureus.CONCLUSIONS: Reusable tourniquets could serve as a potential reservoir for bacterial pathogens.ABBREVIATIONS: CDC = Centers for Disease Control; MRSA = methicillin resistant Staphylococcus aureus; TSB = trypticase soy broth; TSA II = trypticase soy agar with 5% sheep blood; GNI = gram negative identification; BAMC = Brooke Army Medical Center.