RT Journal Article SR Electronic T1 Isolation of multidrug resistant bacterium in urban wildlife. What is in your backyard? JF American Society for Clinical Laboratory Science JO Clin Lab Sci FD American Society of Chemistry and Laboratory Science SP ascls.119.002014 DO 10.29074/ascls.119.002014 A1 Grace Leu-Burke A1 Ty Miller A1 Steven Fowler YR 2019 UL http://hwmaint.clsjournal.ascls.org/content/early/2019/10/09/ascls.119.002014.abstract AB Animal and human interaction encourage zoonotic transmission of microbes resulting in colonization and risk for infectious disease. Studies of individuals working in agriculture show increased cases of Salmonella infection along with a higher colonization of antimicrobial resistant microbes. However risk from wildlife exposure is not fully understood. Alaska’s largest city, Anchorage, is home to approximately one thousand moose migrating into city parks and suburban backyards. Environmental impact of moose fecal contamination could prove to be a public health risk. From November 2018 through February 2019, we collected forty moose fecal samples surveying the Anchorage, Mat-Su Valley region. Utilizing standard microbial techniques isolated bacterium were identified, along with antimicrobial susceptibility testing, using MicroScan™ conventional panels. Colistin resistance on Escherichia coli and phenotypic evaluation for extended spectrum beta-lactamase enzyme in Enterobacteriacea were performed using disc diffusion. Multidrug resistant (MDR) organisms were isolated in 39% of moose fecal samples with 100% containing colistin resistant Escherichia coli. MDR gram negative bacilli identified included Acinetobacter baumanii/haemolyticus, Ewingella americana, Klebsiella ascorbate, Klebsiella ozaenae, Leminorella grimotti, and Pantoea agglomerans. Additionally, Klebsiella isolates expressed phenotypic extended spectrum beta-lactamase enzyme (ESBL). Antibiotic resistant gram positive organisms included methicillin resistant Staphylococcus aureus (MRSA) and linezolid resistant, vancomycin resistant Enterococcus fecalis (VRE). Close proximity of moose in our urban Alaska environment draws attention to their commensal microbiome and potential for infectious transfer to humans. We discovered nearly 40% of moose fecal samples contained one or more multidrug resistant bacterium with several organisms currently identified as a healthcare risk including MRSA, VRE and ESBL producing Klebsiella species. Wildlife exposure to urban landscape, including antibiotic contaminated water, could provide an additional avenue for development of antimicrobial resistant organisms. Further study is indicated to determine potential public health risk of wildlife zoonotic transfer of existing and novel multidrug resistant bacteria.