Table 2.

Estimated concentration of D-dimer in positive-result AS samples

Patient Number	Sample Number	No Dilution	1:2 Dilution	1:4 Dilution	Estimated Concentration
AS patient #1	Sample #1	Negative	N/A	N/A	<1.0 μg/mL
	Sample #2	Positive	Positive	Negative	2–4 μg/mL
	Sample #3	Positive	Positive	Negative	2–4 μg/mL
	Sample #4	Positive	Positive	Negative	2–4 μg/mL
AS patient #2	Sample #1	Negative	N/A	N/A	<1.0 μg/mL
	Sample #2	Negative	N/A	N/A	<1.0 μg/mL
	Sample #3	Negative	N/A	N/A	<1.0 μg/mL
	Sample #4	Negative	N/A	N/A	<1.0 μg/mL
AS patient #3	Sample #1	Positive	Positive	Negative	2–4 μg/mL
	Sample #2	Positive	Positive	Negative	2–4 μg/mL
	Sample #3	Negative	N/A	N/A	<1.0 μg/mL
	Sample #4	Negative	N/A	N/A	<1.0 μg/mL
AS patient #4	Sample #1	Positive	Negative	N/A	1–2 μg/mL
	Sample #2	Positive	Positive	Negative	2–4 μg/mL
	Sample #3	Positive	Positive	Positive	4–8 μg/mL
	Sample #4	Positive	Positive	Positive	4–8 μg/mL

Concentration is estimated based on the highest dilution that produced agglutination.