Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates

Diagn Microbiol Infect Dis. 2009 Apr;63(4):403-8. doi: 10.1016/j.diagmicrobio.2008.12.012. Epub 2009 Feb 18.

Abstract

Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Bacteria / classification*
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Bacterial Infections / diagnosis*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Humans
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • DNA, Bacterial