Research ArticleEducation

Incorporating Hands-on Flow Cytometry Into a Medical Laboratory Science Program Curriculum

Rachel Childs, Cristina Ruffy, Barbara Kraj and Leslie Hoglund
American Society for Clinical Laboratory Science January 2025, 38 (1) 53-58; DOI: https://doi.org/10.29074/ascls.2025003296

Article Figures & Data

Tables

  • Table 1.

    Blackboard online test-item analysis

    QuestionQuestion Type% Students Answering CorrectlyGraded AttemptsAverage ScoreSDStandard Error
    Email the 2 photos taken at the end of lymphocyte subset analysis by flow cytometry […]. In the space below state whether your assigned immunocontrol was…Essay100.00175.000.000.00
    CD4CD8 ratio for patient: Enter the calculated CD4/CD8 ratio for your assigned patient.Essay64.71173.242.130.52
    CD4 population: Your assigned patient’s CD4 population is (depleted/ not depleted):Essay88.24174.421.410.35
    Your assigned patient’s results indicate (type below 1 of the following):Essay82.36174.121.760.43
    CD45: What type of cells will be positive for CD45?Multiple choice86.77174.341.480.36
    Side scatter: Which of these white blood cell populations would have the least side scatter…Multiple choice88.24174.421.670.41
    Overlapping signal: What procedure is required to correct the overlapping signal shown:Multiple choice88.24174.421.670.41
    Median % correct answers:88.24

    Analysis last run July 25, 2019, 8:43 a.m. Blackboard online test-item analysis for flow cytometry homework quiz questions. Only questions relevant to FC shown.

    • Table 2.

      Qualtrics quantitative survey results

      QuestionStrongly Disagree, %Disagree, % (n)Somewhat Disagree, % (n)Neither Agree nor Disagree,
      % (n)      		Somewhat Agree,
      % (n)      		Agree,
      % (n)      		Strongly Agree,
      % (n)
      The flow cytometry hands-on exercise in Clinical Immunology lab (MLS 331) enhanced my understanding of flow cytometry theoretical principles underlying lymphocyte subset analysis.00009.09 (1)27.27 (3)63.64 (7)
      The flow cytometry hands-on exercise in Advanced Hematology (MLS 337) enhanced my understanding of flow cytometry theoretical principles underlying leukemia/lymphoma immunophenotyping.0000045.45 (5)54.55 (6)
      I believe future students would benefit from performing the flow cytometry in immunology and advanced hematology.0009.09 (1)27.27 (3)36.36 (4)27.27 (3)
      I believe the hands-on experience in flow cytometry during the didactic part of the program was relevant to my clinical experience during hospital rotations.09.09 (1)18.18 (2)36.36 (4)9.09 (1)18.18 (2)9.09 (1)
      I believe I am better prepared for practice in the laboratory workforce because of practical experience with flow cytometry.018.18 (2)9.09 (1)18.18 (2)27.27 (3)18.18 (2)9.09 (1)
      I believe I am more competitive on the MLS job market because of practical experience with flow cytometry.018.18 (2)9.09 (1)36.36 (4)9.09 (1)18.18 (2)9.09 (1)

      Extract of responses from the Qualtrics survey.

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      Incorporating Hands-on Flow Cytometry Into a Medical Laboratory Science Program Curriculum
      Rachel Childs, Cristina Ruffy, Barbara Kraj, Leslie Hoglund
      American Society for Clinical Laboratory Science Jan 2025, 38 (1) 53-58; DOI: 10.29074/ascls.2025003296
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