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Research ArticleResearch and Reports

Evaluating Vortexing Times to Disaggregate Platelet Clumps in EDTA Specimens

Gaige Watkins and Brett M. Rice
American Society for Clinical Laboratory Science August 2026, DOI: https://doi.org/10.29074/ascls.2025003325
Gaige Watkins
Augusta University
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Brett M. Rice
Augusta University
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  • For correspondence: brrice{at}augusta.edu
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    Figure 1.

    Complete blood cell count parameter correlations.

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    Table 1.

    Microscopic evaluation for platelet clumps

    GroupPrevortexPostvortexTotal, N
    1 minuteAbsentAbsent23
    Present3
    PresentAbsent4
    Present10
    2 minutesAbsentAbsent18
    Present9
    PresentAbsent0
    Present13
    3 minutesAbsentAbsent20
    Present6
    PresentAbsent4
    Present10
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    Table 2.

    Complete blood cell count parameter comparisons

    ParameterPrevortex, Mean (±SD)Postvortex, Mean (±SD)Pearson Correlation, rSignificance, P Value
    WBC, 103/μL9.3 (±6.1)9.1 (±5.9)0.99<.01
    RBC, 106/μL3.66 (±0.96)3.65 (±0.96)1.00<.01
    HGB, g/dL10.9 (±2.9)10.8 (±2.9)1.00<.01
    HCT, %32.4 (±8.7)32.2 (±8.7)1.00<.01
    PLT, 103/μL181 (±117)199 (±125)0.97<.01

    HCT, hematocrit; HGB, hemoglobin; PLT, platelet; RBC, red blood cell; WBC, white blood cell.

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    American Society for Clinical Laboratory Science: 39 (1)
    American Society for Clinical Laboratory Science
    Vol. 39, Issue 1
    1 Jan 2026
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    Evaluating Vortexing Times to Disaggregate Platelet Clumps in EDTA Specimens
    Gaige Watkins, Brett M. Rice
    American Society for Clinical Laboratory Science Aug 2026, DOI: 10.29074/ascls.2025003325

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    Evaluating Vortexing Times to Disaggregate Platelet Clumps in EDTA Specimens
    Gaige Watkins, Brett M. Rice
    American Society for Clinical Laboratory Science Aug 2026, DOI: 10.29074/ascls.2025003325
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    Keywords

    • CBC - complete blood cell count
    • HCT - hematocrit
    • HGB - hemoglobin
    • Ig - immunoglobulin
    • PPV - positive predictive value
    • PTCP - pseudothrombocytopenia
    • RBC - red blood cell
    • SOP - standard operating procedure
    • WBC - white blood cell
    • hematology
    • complete blood count
    • platelets
    • platelet count
    • EDTA

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