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Research ArticleClinical Practice

An Overview of Venous Blood Gas Analysis

Audrey M. Folsom
American Society for Clinical Laboratory Science August 2026, DOI: https://doi.org/10.29074/ascls.2026003341
Audrey M. Folsom
Medical Laboratory and Radiation Sciences, Arkansas State University
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  • For correspondence: askaggs{at}astate.edu
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  • Fick’s principle formula.
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    Figure 1.

    Fick’s principle formula.

  • Oxygen content calculation.
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    Figure 2.

    Oxygen content calculation.

Tables

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    Table 1.

    VBG reference intervals for pH, pCO2, and HCO3−

    VBG ParameterStudy VBG Reference Interval
    pH7.30–7.43
    pCO2, mm Hg38–58
    Formula, mmol/L22–30

    Source: Adapted from Ress et al.18 Abbreviations: HCO3−, bicarbonate; pCO2, partial pressure of CO2; VBG, venous blood gas.

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      Table 2.

      Meta-analysis theoretical VBG reference intervals for pH, pCO2, and HCO3−

      VBG ParameterTheoretical VBG Reference
      Interval (Meta-Analysis)
      pH7.32–7.42
      pCO2, mm Hg
       Male39–52
       Female36–49
      Formula, mmol/L23–27

      Source: Adapted from Ress et al.18 Abbreviations: HCO3−, bicarbonate; pCO2, partial pressure of CO2; VBG, venous blood gas.

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        Table 3.

        Mathematical models for pCO2 conversion

        ModelFormula
        Zeserson model (mm Hg)23peCO2 = pvCO2 − 4.8
        Farkas model (mm Hg)22peCO2 = pvCO2 − (0.22 × (93 − svO2))
        Lemoël model (mm Hg)21peCO2 = pvCO2 − (0.3 × (75 − svO2))

        Abbreviations: pCO2, partial pressure of CO2; peCO2, estimated pCO2; pvCO2, venous pCO2; svO2, venous functional oxygen saturation.

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        In this issue

        American Society for Clinical Laboratory Science: 38 (1)
        American Society for Clinical Laboratory Science
        Vol. 38, Issue 1
        1 Jan 2025
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        An Overview of Venous Blood Gas Analysis
        Audrey M. Folsom
        American Society for Clinical Laboratory Science Aug 2026, DOI: 10.29074/ascls.2026003341

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        An Overview of Venous Blood Gas Analysis
        Audrey M. Folsom
        American Society for Clinical Laboratory Science Aug 2026, DOI: 10.29074/ascls.2026003341
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        • Article
          • ABSTRACT
          • INTRODUCTION
          • METHODS
          • THE ADVANTAGE OF VBG ANALYSIS
          • TRADITIONAL USE OF VBGs: ABG/VBG PAIRED ANALYSES
          • NEWER VBG APPLICATIONS IN VARIOUS CLINICAL CONDITIONS AND SETTINGS
          • COLLECTION AND TRANSPORT CONSIDERATIONS
          • ANALYSIS AND INTERPRETATION
          • REFERENCES
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        Keywords

        • Δpv-aCO2 - venous-to-arterial carbon dioxide pressure difference
        • ABG - arterial blood gas
        • cVBG - central venous blood gas
        • CVC - central venous catheter
        • DKA - diabetic ketoacidosis
        • ED - emergency department
        • HCO3− - bicarbonate
        • ICU - intensive care unit
        • pCO2 - partial pressure of CO2
        • pO2 - partial pressure of O2
        • sO2 - functional oxygen saturation
        • VBG - venous blood gas
        • VO2 - volume of oxygen consumption
        • blood gas analysis
        • venous blood gas
        • acid–base balance
        • hypercarbia

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