RT Journal Article
SR Electronic
T1 Polyoxometalate Induces Susceptibility of Methicillin-Resistant Staphylococcus aureus to Oxacillin
JF American Society for Clinical Laboratory Science
JO Clin Lab Sci
FD American Society of Chemistry and Laboratory Science
SP ascls.118.000760
DO 10.29074/ascls.118.000760
A1 Wilkinson, Katie
A1 Wilkinson, Tyler
A1 Adamson, Candace
A1 Herzog, Timothy
A1 Covey, Tracy
A1 Lloyd, Barry
A1 Nicholaou, Matthew
YR 2018
UL http://hwmaint.clsjournal.ascls.org/content/early/2018/09/04/ascls.118.000760.abstract
AB Transition metal inorganic compounds, also known as polyoxometalates (POM), have many biological applications such as antiviral, antitumor, and antibacterial therapies. The objective of this study was to determine if the POM α-K6P2W18O62 · 14H2O · 14H2O could overcome the resistance of methicillin resistant Staphylococcus aureus (MRSA). To determine the minimal inhibitory concentration (MIC) and possible synergistic effects, multiple dilutions of oxacillin and POM were combined with inoculums of MRSA. Susceptibility (MIC ≤ 0.25 µg/mL oxacillin) was achieved at a concentration of 5 µM of α-K6P2W18O62 · 14H2O. The POM enhanced the efficacy of oxacillin, and additionally, the POM showed low toxicity to mammalian cell cultures in vitro. The effect of the POM on mecA gene transcription was assessed using reverse transcriptase quantitative PCR (RT-qPCR) and showed a reduction in mRNA transcription at effective POM doses, but increased transcription was observed at higher POM oxacillin doses. This pilot study illustrates that α-K6P2W18O62 · 14H2O could be used in conjunction with oxacillin. However, further testing would need to be completed.