PT  - JOURNAL ARTICLE
AU  - Wilkinson, Katie
AU  - Wilkinson, Tyler
AU  - Adamson, Candace
AU  - Herzog, Timothy
AU  - Covey, Tracy
AU  - Lloyd, Barry
AU  - Nicholaou, Matthew
TI  - Polyoxometalate Induces Susceptibility of Methicillin-Resistant Staphylococcus Aureus to Oxacillin
AID  - 10.29074/ascls.2018000760
DP  - 2018 Apr 01
TA  - American Society for Clinical Laboratory Science
PG  - 59--64
VI  - 31
IP  - 2
4099  - http://hwmaint.clsjournal.ascls.org/content/31/2/59.short
4100  - http://hwmaint.clsjournal.ascls.org/content/31/2/59.full
SO  - Clin Lab Sci2018 Apr 01; 31
AB  - Transition metal inorganic compounds, also known as polyoxometalates (POM), have many biological applications, such as antiviral, antitumor, and antibacterial therapies. The objective of this study was to determine if the POM α-K6P2W18O62 · 14H2O could overcome the resistance of methicillin-resistant Staphylococcus aureus (MRSA). To determine the minimal inhibitory concentration (MIC) and possible synergistic effects, multiple dilutions of oxacillin and POM were combined with inoculums of MRSA. Susceptibility (MIC ≤ 0.25 µg/mL oxacillin) was achieved at a concentration of 5 µM of α-K6P2W18O62 · 14H2O. The POM enhanced the efficacy of oxacillin and additionally showed low toxicity to mammalian cell cultures in vitro. The effect of the POM on mecA gene transcription was assessed using reverse transcriptase–quantitative polymerase chain reaction and showed a reduction in messenger RNA transcription at effective POM doses; however, increased transcription was observed at higher POM oxacillin doses. This pilot study illustrates that α-K6P2W18O62 · 14H2O could be used in conjunction with oxacillin. However, further testing needs to be completed.