<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Randolph, Tim R.</style></author><author><style face="normal" font="default" size="100%">Le, Austin</style></author><author><style face="normal" font="default" size="100%">DeMond, Jeffery</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">A Modified Sodium Metabisulfite Method to Distinguish Sickle Cell Disease from Sickle Cell Trait for Use in Underdeveloped Countries</style></title><secondary-title><style face="normal" font="default" size="100%">American Society for Clinical Laboratory Science</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2019-07-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">93-93</style></pages><doi><style  face="normal" font="default" size="100%">10.29074/ascls.2019002030</style></doi><volume><style face="normal" font="default" size="100%">32</style></volume><issue><style face="normal" font="default" size="100%">3</style></issue><abstract><style  face="normal" font="default" size="100%">The objective of this study was to develop a simple, inexpensive, and rapid confirmatory test using a sodium metabisulfite microscopic method to distinguish AS from SS genotype in patients with positive test results for hemoglobin S. Equal volumes of de-identified EDTA blood and 2% sodium metabisulfite were mixed, placed on a microscope slide with coverslip, and observed for sickling at 30-minute intervals over 3 hours. Sickle cells were enumerated per 200 red blood cells (RBCs) under 100x oil immersion and placed into 4 Likert categories (1+ to 4+) based on degree of sickling. In AS samples, 2+ and 3+ sickle cells rose most rapidly, and 4+ sickle cells showed the steepest rise in the SS samples. Based on these data, the number of 4+ sickle cells were counted every 30-minutes over 3 hours in 5 AS and 28 SS samples at 37°C. The mean numbers of 4+ sickle cells in AS samples were 4.75 per 200 RBCs at 2 hours and 17.75 per 200 RBCs at 3 hours. SS samples yielded 78.29 per 200 RBCs at 2 hours and 115.43 per 200 RBCs at 3 hours. Two-hour incubation showed a statistical difference (P = 0.00024) among groups in the shortest time and may be used to distinguish SS from AS genotypes. More testing is needed to determine cut points to distinguish genotype.</style></abstract></record></records></xml>