RT Journal Article
SR Electronic
T1 An Interdisciplinary Enrichment Laboratory on Lectin Production
JF American Society for Clinical Laboratory Science
JO Clin Lab Sci
FD American Society of Chemistry and Laboratory Science
SP 52
OP 56
DO 10.29074/ascls.2021003159
VO 34
IS 4
A1 Rhees, Justin R.
A1 Harley, Suzanne M.
YR 2021
UL http://hwmaint.clsjournal.ascls.org/content/34/4/52.abstract
AB In order to provide students with an appreciation for how lectin reagents have historically been obtained, the medical laboratory science (MLS) immunohematology instructor arranged with a botany instructor to provide an enrichment laboratory. MLS students observed lectin extraction and processing of extract from Pisum sativum seeds to purify Pisum lectin by affinity chromatography. Students were provided the opportunity to use a NanoDrop Lite Spectrophotometer to monitor the presence of the lectin during chromatography. This activity provided students the opportunity to see how various analytical methods are used outside the MLSs. The results of an anonymous postactivity survey indicated the respondents would recommend the activity for future students and were interested in seeing applications for analytic methods outside the MLS profession. The quiz results indicated good retention of the material learned approximately 6 months after the activity was conducted.