PT - JOURNAL ARTICLE AU - Kemp, Chesley AU - Rice, Brett TI - Comparison of Laboratory Scientist and CellaVision DM9600 Classification of Reactive Lymphocytes AID - 10.29074/ascls.2022003211 DP - 2024 Nov 14 TA - American Society for Clinical Laboratory Science 4099 - http://hwmaint.clsjournal.ascls.org/content/early/2024/11/15/ascls.2022003211.short 4100 - http://hwmaint.clsjournal.ascls.org/content/early/2024/11/15/ascls.2022003211.full AB - The CellaVision DM9600 (CV) is an automated digital microscopy system that performs peripheral blood cell differentials. Manual differentials are prone to variable results between laboratory scientists (LSs). The CV may reduce this subjectivity, especially in the classification of reactive lymphocytes (RLs). The first aim was to investigate the correlation between CV and LS classification of RLs. The second aim was to determine whether the LS performing the reclassification affects the difference in the number of RLs classified between the CV and LS. The sample identified 3925 CV differentials completed between January 2018 and July 2019 with RLs identified by the CV, LS, or both. A related-samples Wilcoxon signed-rank test showed a statistically significant difference (P < .001) between the median number of RLs classified by CV and LSs. Spearman’s Rho showed no statistically significant correlation (P = .455). An independent-samples Kruskal-Wallis test showed a significant difference between LSs (P < .001). Dunn analysis further described the variability between LSs. Although there is no real correlation between CV and LSs’ classification of RLs, the difference between CV and LS is influenced by which LS is performing the analysis. Future studies may investigate factors influencing RL classification among LSs.