mNGS steps and needed skillsets
Step of mNGS | Associated/Needed Skills | Automated or Streamlined Solutions Available |
|---|---|---|
| Nucleic acid extraction | 1. Knowledge of molecular workflow 2. Molecular sample handling techniques | Automation |
| Complementary DNA creation (for RNA libraries) | Same as previously mentioned, including handling and manipulation of RNA | None |
| Library preparation | 1. Knowledge of molecular workflow 2. Molecular/micropipetting techniques 3. Understanding of NGS methodology and calculations 4. Critical thinking/troubleshooting of NGS preparation steps | Automation |
| Instrument operation | Experience or proper training | None |
| Quality control | Critical thinking/troubleshooting of NGS preparation steps | None |
| Read/data analysis | All of the previously mentioned items, including bioinformatics training and programming knowledge | Web-based commercial pipeline |
| Data interpretation | 1. All of the previously mentioned items, including experience and involvement in the test validation 2. Medical Director review, chart review, consultation, and sign-out | Establishment of positive/negative pipeline criteria |
Abbreviations: mNGS, metagenomic NGS; NGS, next-generation sequencing.