Effects of Glucosamine and Celadrin on Platelet Function

Abstract

OBJECTIVE: The purpose of this study was to determine the effects of glucosamine and celadrin on platelet function.

DESIGN: Baseline values were determined on the Chronolog 570VS platelet aggregometer with whole blood aggregation impedance readings using 2 different concentrations of ADP (5μM, 10μM), collagen (1μg/mL), arachidonic acid (0.5mM/L) and an Accumetrics whole blood platelet aggregation cartridge assay for P2Y12 receptors were obtained from 24 healthy volunteers. These subjects then took the suggested doses of Glucosamine with Celadrin (Stockbridge Naturals) as advertised (estimated 1500mg daily) for 2 weeks. Platelet aggregation analyses, as described above, were obtained after treatment. Statistics performed via a McNemar test.

MAIN OUTCOME: Five of twenty-four subjects had at least a 20% difference in whole blood aggregation using the 5μM concentration of ADP. A total of 6 and 7 subjects also showed a significant difference in platelet aggregation with administration of collagen and arachidonic acid, respectively. No significant differences were found with Accumetrics assay for P2Y12 in any of the subjects.

CONCLUSION: Glucosamine and celadrin may inhibit platelet aggregation in some individuals via aspirin-like effects as well as inhibition of ADP receptor P2Y1 but not P2Y12.

ABBREVIATIONS: ADP = adenosine diphosphate, NSAID = non-steroidal anti-inflammatory drug, EDTA = ethylenediaminetetraacetic acid, WBA = whole blood aggregation, CBC = complete blood count, PGE = prostaglandin-E