CLS Meets the Aquaporin Family: Clinical Cases Involving Aquaporin Systems

Wayne Gade; Brooke Robinson

doi:10.29074/ascls.19.2.80

This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.

Wayne Gade, PhD⇑
1. is of the Departments of Clinical Laboratory Science and Chemistry at the University of Illinois at Springfield, Springfield IL
Brooke Robinson
1. holds a Bachelor of Science in Biology from the University of Illinois at Springfield, Springfield IL

Address for correspondence: Wayne Gade PhD, Departments of Clinical Laboratory Science and Chemistry, HSB 314, University of Illinois at Springfield, One University Place, Springfield IL 62703. (217) 206-7349, (217) 206-6162 (fax). gade.wayne{at}uis.edu

Extract

Virtually all human cells incorporate aquaporins, or water channel proteins, into their cell membrane. Indeed, many cells produce several aquaporins, each adapted for a specific physiologic function. Thus, it is not surprising that aquaporin malfunctions are associated with numerous important clinical conditions. This article describes the clinical aspects of malfunctions in aquaporins or their regulation.

Although water can diffuse across biological membranes (osmosis) without the aid of a transport system, researchers had predicted for decades that rapid reabsorption by renal tubule cells must be aided by a channel or pore. Yet, not until the 1990s were the first members of the aquaporin (AQP) family identified. Led by Dr. Peter Agre, recipient of the 2003 Nobel Prize in Chemistry, researchers have since amassed an astounding amount of information about AQPs and their function. For example, the flow rate of water through AQP1 is an extraordinary three billion water molecules per second per aquaporin channel, while a relative trickle of water crosses the hydrophobic lipid bilayer of cell membranes devoid of AQPs.

Our understanding of renal physiology and pathophysiology has advanced greatly as we account for the subtle implications of various AQP systems. For example, nephrogenic diabetes insipidus (NDI), the inability to produce concentrated urine, can result from several different malfunctions in the hormonally controlled AQP2 system. The list of diseases known to involve AQPs now includes: early onset of cataracts, Sjogren's syndrome, cerebral and pulmonary edemas, cirrhotic liver development of ascites, and congestive heart failure (CHF).

The first aquaporin, originally called…

ABBREVIATIONS: ADH = anti-diuretic hormone; AQP = aquaporin; cAMP= cyclic adenosine monophosphate; CDI = central diabetes insipidus; CHF = congestive heart failure; MIP = major intrinsic protein; NDI = nephrogenic diabetes insipidus; PCT = proximal convoluted tubule; RT-PCR = reverse transcription - polymerase chain reaction; SS = Sjogren's syndrome; SIADH = syndrome of inappropriate ADH secretion.