Skip to main content

Main menu

  • Home
  • Content
    • Current
    • Ahead of print
    • Archive
  • Info for
    • Authors
    • Reviewers
  • About Us
    • About Us
    • Conflict of Interest
    • Informed Consent
    • Human and Animal Rights
  • More
    • Alerts
    • Feedback
    • Folders
  • ascls.org
    • ascls.org

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
American Society for Clinical Laboratory Science
  • ascls.org
    • ascls.org
  • My alerts
  • Log in
  • My Cart
American Society for Clinical Laboratory Science

Advanced Search

  • Home
  • Content
    • Current
    • Ahead of print
    • Archive
  • Info for
    • Authors
    • Reviewers
  • About Us
    • About Us
    • Conflict of Interest
    • Informed Consent
    • Human and Animal Rights
  • More
    • Alerts
    • Feedback
    • Folders
  • Follow ASCLS on Twitter
  • Visit ASCLS on Facebook
  • Follow ASCLS on Instagram
  • RSS Feed
Research ArticleResearch and Reports

Duration of Loxosceles reclusa Venom Detection by ELISA from Swabs

David L. McGlasson, Jonathon A. Green, William V. Stoecker, James L. Babcock and David A. Calcara
American Society for Clinical Laboratory Science October 2009, 22 (4) 216-222; DOI: https://doi.org/10.29074/ascls.22.4.216
David L. McGlasson
is clinical research scientist, Wilford Hall Medical Center, Lackland AFB, TX
MS, MLS
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: David.McGlasson@lackland.af.mil
Jonathon A. Green
is an associate professor, Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO
PhD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William V. Stoecker
is president and CEO, Stoecker & Associates, Rolla, MO
MS, MD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
James L. Babcock
is staff pathologist, Ozark Pathology Associates, Mountain Home, AR
MD, PhD
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David A. Calcara
is a medical student, Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO
BS
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • PDF
Loading

This article requires a subscription to view the full text. If you have a subscription you may use the login form below to view the article. Access to this article can also be purchased.

  1. David L. McGlasson, MS, MLS⇑
    1. is clinical research scientist, Wilford Hall Medical Center, Lackland AFB, TX
  2. Jonathon A. Green, PhD
    1. is an associate professor, Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO
  3. William V. Stoecker, MS, MD
    1. is president and CEO, Stoecker & Associates, Rolla, MO
  4. James L. Babcock, MD, PhD
    1. is staff pathologist, Ozark Pathology Associates, Mountain Home, AR
  5. David A. Calcara, BS
    1. is a medical student, Department of Animal Sciences, University of Missouri-Columbia, Columbia, MO
  1. Address for Correspondence: David L. McGlasson, MS, MLS, 59th Clinical Research Division, CPSG/SGVUL, 2200 Bergquist Drive, Bldg. 4430, Lackland AFB, TX 78236-9908, Tel: 210-292-6555 Fax: 210-292-2897, David.McGlasson{at}lackland.af.mil

Abstract

BACKGROUND: Diagnosis of Loxosceles reclusa envenomations is currently based upon clinical presentation. An enzyme-linked immunosorbent assay (ELISA) can detect surface Loxosceles venom at the envenomation site, allowing diagnostic confirmation. The length of time that venom on the skin is recoverable non-invasively is unknown.

MATERIALS AND METHODS: To investigate duration of recoverable venom antigen, whole venom and fractionated sphingomyelinase D venom aliquots were injected subcutaneously in New Zealand White rabbits. Cotton and Dacron swabs were compared for venom recovery over a 21-day period using a surface swab technique.

RESULTS: Significant amounts of Loxosceles reclusa antigen were found on the surface of rabbit skin after experimental injection of whole venom and sphingomyelinase D. The duration of recoverable antigen using this experimental model appears to be at least two weeks and as long as 21 days in some cases.

CONCLUSIONS: Because the duration of the recoverable antigen is seen to be at least two weeks, the ELISA venom test appears capable of detecting venom on most patients presenting with Loxosceles envenomations. This detection system will allow the physician more accurate determination of whether the lesion is from a brown recluse spider or some other agent that can cause this type of necrotic ulcer.

  • ©Copyright 2009 American Society for Clinical Laboratory Science Inc. All rights reserved.
PreviousNext
Back to top

In this issue

American Society for Clinical Laboratory Science: 22 (4)
American Society for Clinical Laboratory Science
Vol. 22, Issue 4
Fall 2009
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on American Society for Clinical Laboratory Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Duration of Loxosceles reclusa Venom Detection by ELISA from Swabs
(Your Name) has sent you a message from American Society for Clinical Laboratory Science
(Your Name) thought you would like to see the American Society for Clinical Laboratory Science web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Duration of Loxosceles reclusa Venom Detection by ELISA from Swabs
David L. McGlasson, Jonathon A. Green, William V. Stoecker, James L. Babcock, David A. Calcara
American Society for Clinical Laboratory Science Oct 2009, 22 (4) 216-222; DOI: 10.29074/ascls.22.4.216

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Request Permissions
Share
Duration of Loxosceles reclusa Venom Detection by ELISA from Swabs
David L. McGlasson, Jonathon A. Green, William V. Stoecker, James L. Babcock, David A. Calcara
American Society for Clinical Laboratory Science Oct 2009, 22 (4) 216-222; DOI: 10.29074/ascls.22.4.216
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • Google Scholar

Cited By...

  • A Brief Overview of the Toxic Sphingomyelinase Ds of Brown Recluse Spider Venom and Other Organisms and Simple Methods To Detect Production of Its Signature Cyclic Ceramide Phosphate
  • Validation of a colorimetric LAMP to detect Loxosceles experimental envenomation
  • Google Scholar

More in this TOC Section

  • Barriers to Implementation of Quality Management Systems in Laboratories: Lessons from the Southern Africa TB Health Systems Project
  • Polyagglutination: Lectin Isolation for T-Activated Red Cells
  • Strength of Direct Antiglobulin Test as an Indicator of Disease Severity in Autoimmune Hemolytic Anemia
Show more Research and Reports

Similar Articles

© 2025 The American Society for Clinical Laboratory Science

Powered by HighWire