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Research ArticleResearch and Reports

Development of a Microscopic Method to Identify Hemoglobin C Conditions for Use in Developing Countries

Kayla L. Schmidt and Tim R. Randolph
American Society for Clinical Laboratory Science April 2019, 32 (2) 61-66; DOI: https://doi.org/10.29074/ascls.2019001412
Kayla L. Schmidt
Saint Louis University
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Tim R. Randolph
Saint Louis University
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    Figure 1.

    Shows HbC crystals under 100x oil immersion on a bright field microscope. Two RBCs highlighted with the white box do not have HbC crystals.

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    Figure 2.

    Crystal formation in various concentrations of DPBS versus NaCl. * = statistical significance.

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    Figure 3.

    Crystal formation at different incubation times in DPBS. * = statistical significance.

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    Figure 4.

    Crystal formation in different incubation temperatures in DPBS. * = statistical significance.

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    Table 1.

    Cost analysis for microscopic HbC method: major consumable reagents and materials needed to perform the microscopic HbC method with costs/unit, costs/test, and cost/150 tests


    Product
    Unit SizeUnit CostCost/TestCost/150 Test
    DPBS (10X concentrate)1 L$36.00$0.036$5.40
    New methylene blue250 mL$37.30$0.012$1.80
    Parafilm125’ x 4”$28.50$0.038$5.70
    Total cost$101.80$0.086$12.90
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American Society for Clinical Laboratory Science: 32 (2)
American Society for Clinical Laboratory Science
Vol. 32, Issue 2
1 Apr 2019
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Development of a Microscopic Method to Identify Hemoglobin C Conditions for Use in Developing Countries
Kayla L. Schmidt, Tim R. Randolph
American Society for Clinical Laboratory Science Apr 2019, 32 (2) 61-66; DOI: 10.29074/ascls.2019001412

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Development of a Microscopic Method to Identify Hemoglobin C Conditions for Use in Developing Countries
Kayla L. Schmidt, Tim R. Randolph
American Society for Clinical Laboratory Science Apr 2019, 32 (2) 61-66; DOI: 10.29074/ascls.2019001412
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Keywords

  • ANOVA - analysis of variance
  • DPBS - Dulbecco’s phosphate buffered saline
  • HbA - hemoglobin A
  • HbAA - hemoglobin AA
  • HbAC - hemoglobin A disease
  • HbC - hemoglobin C
  • HbCC - hemoglobin C disease
  • HbS - sickle cell hemoglobin
  • HbSC - hemoglobin SC
  • HbSS - sickle cell anemia
  • HE - hemoglobin electrophoresis
  • HPLC - high-performance liquid chromatography
  • IEF - isoelectric focusing
  • IRB - Institutional Review Board
  • NaCl - sodium chloride
  • RBC - red blood cell

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