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Research ArticleResearch and Reports

Polyagglutination: Lectin Isolation for T-Activated Red Cells

Jesus Rebolledo, Alexander Fishburn, Arren Hill, Sandy Wortman, Matthew Nicholaou and Justin R. Rhees
American Society for Clinical Laboratory Science April 2022, 35 (2) 29-34; DOI: https://doi.org/10.29074/ascls.2020003147
Jesus Rebolledo
Weber State University
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Alexander Fishburn
Weber State University
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Arren Hill
Weber State University
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Sandy Wortman
Carter BloodCare
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Matthew Nicholaou
Weber State University
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Justin R. Rhees
Weber State University
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    Figure 1.

    Survey questions—PA testing occurrences. PA, polyagglutination.

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    Figure 2.

    Survey questions—PA testing storage and expiration. IRL, immunohematology reference laboratory; PA, polyagglutination; QC, quality control.

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    Table 1.

    Monthly hemagglutination reactions for positive and negative QC cells

    Month 1Month 2Month 3Month 4
    Positive control4+4+4+NP*
    Negative control0000

    Abbreviations: NP, not performed.

    *T-activated positive QC cells were hemolyzed during the fourth month of testing, so a 3%–5% cell suspension could not be made.

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    American Society for Clinical Laboratory Science: 35 (2)
    American Society for Clinical Laboratory Science
    Vol. 35, Issue 2
    1 Apr 2022
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    Polyagglutination: Lectin Isolation for T-Activated Red Cells
    Jesus Rebolledo, Alexander Fishburn, Arren Hill, Sandy Wortman, Matthew Nicholaou, Justin R. Rhees
    American Society for Clinical Laboratory Science Apr 2022, 35 (2) 29-34; DOI: 10.29074/ascls.2020003147

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    Polyagglutination: Lectin Isolation for T-Activated Red Cells
    Jesus Rebolledo, Alexander Fishburn, Arren Hill, Sandy Wortman, Matthew Nicholaou, Justin R. Rhees
    American Society for Clinical Laboratory Science Apr 2022, 35 (2) 29-34; DOI: 10.29074/ascls.2020003147
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    Keywords

    • AABB - Association for the Advancement of Blood & Biotherapies
    • ABC - America’s Blood Centers
    • EDTA - ethylenediaminetetraacetic acid
    • g - gravities
    • gm - grams
    • IRB - Institutional Review Board
    • IRL - immunohematology reference laboratory
    • PA - polyagglutination
    • PBS - phosphate-buffered saline
    • QC - quality control
    • RBC - red blood cell
    • RPM - revolutions per minute
    • RT - room temperature
    • hemagglutination
    • lectins
    • in vitro clinical test
    • laboratory-developed test
    • in vitro diagnostics

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