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Research ArticleResearch and Reports

Innovative Approach to Moderating Risk of Nosocomial Infection During Anesthesia

Thomas Corey Davis, Beverly George Gay, Melissa Jamerson, Sarah A. Marrs, Ronsard Daniel and Chuck J. Biddle
American Society for Clinical Laboratory Science April 2023, DOI: https://doi.org/10.29074/ascls.2020002246
Thomas Corey Davis
Virginia Commonwealth University
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Beverly George Gay
Virginia Commonwealth University
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Melissa Jamerson
Virginia Commonwealth University
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Sarah A. Marrs
Virginia Commonwealth University
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Ronsard Daniel
Virginia Commonwealth University
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Chuck J. Biddle
Virginia Commonwealth University
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  • Article
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Article Figures & Data

Figures

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  • Figure 1.
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    Figure 1.

    Vectors of microbial contamination in the AW.

  • Figure 2.
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    Figure 2.

    A typical anesthesia machine with architectural/design complexity, making cleaning and disinfection between surgical procedures very challenging.

  • Figure 3.
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    Figure 3.

    Location of condom-like protective covers: (A) vaporizer control dials, (B) oxygen flowmeter control, (C) electrical medical record control mouse, and (D) breathing circuit pressure control valve (APL valve).

  • Figure 4.
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    Figure 4.

    Preliminary study to assess wrap barrier effectiveness. Growth on SBA after 48-hour incubation (A) outside of plastic cover after inoculation with S epidermis, (B) inside of plastic cover after inoculation with S epidermis, and (C) knob covered after inoculation with S epidermis.

  • Figure 5.
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    Figure 5.

    Chronology of culturing in the intervention and control room cases.

Tables

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    Table 1.

    CFU range for control group vs intervention group


    Location
    Control Group CFU Range (CFU/mL)Control Group Median CFU CountIntervention Group CFU Range (CFU/mL)Intervention Group Median CFU Count
    APL valve0–15008.500–900.50*
    Oxygen flow valve0–39 5004.500–6600.50*
    Vaporizer dials0–2402.000–1400.00*
    Computer mouse0–32010.000–800.50*

    *Significant difference (P < 0.01) in CFU/mL between the control (uncovered condition) and intervention (covered condition).

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      Table 2.

      List of bacteria cultured from the anesthesia machine

      LocationControl Group OrganismsIntervention Group Organisms
      APL valveCoNS, Streptococcus species, Micrococcus speciesCoNS, Bacillus species, Streptococcus species, Corynebacterium species, Micrococcus species
      Oxygen flow knobsCoNS, Micrococcus species, Streptococcus species, Bacillus species, S aureusCoNS, Streptococcus species, Bacillus species
      Vaporizer dialsCoNS, Bacillus species, Streptococcus speciesCoNS, Micrococcus species
      Computer mouseCoNS, Micrococcus species, Corynebacterium species, Streptococcus species, Bacillus speciesCoNS, Streptococcus species, Bacillus species, Micrococcus species

      CoNS, coagulase-negative Staphylococcus species.

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      American Society for Clinical Laboratory Science: 37 (2)
      American Society for Clinical Laboratory Science
      Vol. 37, Issue 2
      1 Apr 2024
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      Innovative Approach to Moderating Risk of Nosocomial Infection During Anesthesia
      Thomas Corey Davis, Beverly George Gay, Melissa Jamerson, Sarah A. Marrs, Ronsard Daniel, Chuck J. Biddle
      American Society for Clinical Laboratory Science Apr 2023, DOI: 10.29074/ascls.2020002246

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      Innovative Approach to Moderating Risk of Nosocomial Infection During Anesthesia
      Thomas Corey Davis, Beverly George Gay, Melissa Jamerson, Sarah A. Marrs, Ronsard Daniel, Chuck J. Biddle
      American Society for Clinical Laboratory Science Apr 2023, DOI: 10.29074/ascls.2020002246
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      Keywords

      • ADM - anesthesia delivery machine
      • APL - adjustable pressure-limiting
      • AW - anesthesia workstation
      • CFU - colony-forming unit
      • EMR - electronic medical record
      • HAI - health care–associated infection
      • IV - intravenous
      • IRB - Institutional Review Board
      • OR - operating room
      • PYR - pyrrolidonyl arylamidase
      • SBA - sheep blood agar
      • VCUMC - Virginia Commonwealth University Medical Center

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