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Development of a Procedure to Resolve Daratumumab Interference in Pretransfusion Testing

Kaitlin Walsh, Tara C. Moon, Troy Dang and Caroline Immel
American Society for Clinical Laboratory Science April 2023, DOI: https://doi.org/10.29074/ascls.2020002600

Article Figures & Data

Tables

  • Table 1.

    Summary of antibody screening of patients treated with DARA





    Patient    		Antibody Screen With Untreated Reagent Cells (AHG Phase)Antibody Screen With DTT-treated Reagent Cells (AHG Phase)



    Autocontrol
    1SC I1+SC INegativeNegative
    SC II1+SC IINegative
    SC III1+SC IIINegative
    2SC I1+SC INegativeNegative
    SC IINegativeSC IINegative
    SC III1+SC IIINegative
    3SC INegativeSC INegativeNegative
    SC IIWeakSC IINegative
    SC III1+SC IIINegative
    4SC I1+SC INegativeNegative
    SC II1+SC IINegative
    SC III1+SC IIINegative

    SC, screening cell.

    • Table 2.

      Quality-control results with anti-K, 45-minute incubation with DTT

      Anti-K PlusReactionInterpretation
      Positive control (K+k+)4+Positive
      Negative control (K−k+)0Negative
      DTT-treated SC II (K+k+)0Negative

      SC, screening cell.

      • Table 3.

        Quality-control results with anti-K, 30-minute incubation with DTT

        Anti-K PlusReactionInterpretation
        Positive control (K+k+)3+Positive
        Negative control (K−k+)0Negative
        DTT-treated SC II (K+k+)WeakPositive

        SC, screening cell.

        • Table 4.

          Reagent cost breakdown


          Reagent
          Reagent Cost          		Unit
          Volume (mL)          		Drops
          per Unit          		Cost
          per Drop          		Number
          of Drops Used          		Volume
          Used (mL)
          Total Cost
          DTT$53.58240$1.4012N/A$16.80
          SC I$60.0010200$0.307N/A$2.10
          SC II$60.0010200$0.307N/A$2.10
          SC III$60.0010200$0.307N/A$2.10
          PEG$21.0010200$0.1114N/A$1.47
          Anti-immunoglobulin G$28.5010200$0.1414N/A$1.96
          Check cells$32.0010200$0.163N/A$0.48
          Anti-K antisera$250.005100$2.503N/A$7.50
          K+ panel cell$30.005100$0.301N/A$0.30
          K− panel cell$30.005100$0.301N/A$0.30
          PBS$20.001000N/AN/AN/A226$5.42
          TotalN/AN/AN/AN/AN/AN/A$40.53

          N/A, not applicable; SC, screening cell.

          • Table 5.

            Supply cost breakdown

            SupplyCost per Individual
            Supply
            Number of Supplies Used
            Total Cost
            12×75-mm test tubes$0.0213$0.26
            Transfer pipets$0.0321$0.63
            TotalN/AN/A$0.89

            N/A, not applicable.

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            Development of a Procedure to Resolve Daratumumab Interference in Pretransfusion Testing
            Kaitlin Walsh, Tara C. Moon, Troy Dang, Caroline Immel
            American Society for Clinical Laboratory Science Apr 2023, DOI: 10.29074/ascls.2020002600
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