Cinnamaldehyde Inhibits MRSA Biofilm Formation and Reduces Cell Viability

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Figure 1.
Cinnamaldehyde Inhibits Biofilm Formation by MRSA. Clinical isolates of MRSA were assessed for effect of cinnamaldehyde (at various concentrations: 0 μM, 10 μM, 25 μM, 50 μM, 100 μM) on biofilm formation (37°C, 1 h or 2 h incubation periods). All reactions were performed in triplicate and four strains of MRSA were assessed (n=4). Biofilm was stained, solubilized, assessed spectrophotometrically (A₅₉₀) and reported as crystal violet-stained biofilm (Biofilm (590 nm)). * = p<0.01 as determined by ANOVA followed by Tukey post hoc analysis.
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Figure 2.
Effects of Cinnamaldehyde on Viability of S. aureus. Number of colony-forming units/ml (CFU/ml) was determined by serial dilution of bacteria in broth (TSB) followed by cinnamaldehyde addition (0 μM, 10 μM, 25 μM, 50 μM, 100 uM), incubation (2 h, 37°C), plating of broth bacteria (10⁶ and 10⁸) onto agar plates, incubation (overnight, 37°C), and counting of individual colonies that grew. Number of colonies counted in the dilution having countable numbers of bacteria (10⁸ dilution factor) was used for statistical analyses and graphically displayed since the other dilutions had CFU/ml that were too numerous to count (>500 colonies). MRSA results were displayed in Panel A (n=3). MSSA (n=3) and S. aureus (n=6) results were displayed in Panel B.