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Research_ArticleResearch Article

The Role of Hyperglycemia in CD4 T Cell Survival and Differentiation

Naomi Iwai, Catherine Steib, Amanda Marzo and Nadine M Lerret
American Society for Clinical Laboratory Science May 2018, ascls.118.000331; DOI: https://doi.org/10.29074/ascls.118.000331
Naomi Iwai
Rush University
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Catherine Steib
Rush University
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Amanda Marzo
Rush University
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Nadine M Lerret
Rush University
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  1. Naomi Iwai
  2. Catherine Steib
  3. Amanda Marzo
  4. Nadine M Lerret1
  1. Rush University
  1. ↵* Corresponding author; email: nadine_lerret{at}rush.edu

Abstract

The major driving force in the pathogenesis of pre-diabetes and Type 2 Diabetes is thought to be chronic and low grade systemic inflammation. Immune cells are key players in the induction of this chronic inflammation; however, the type of immune cells and mechanisms underlying the rapidly progressing pathogenesis of this disease are unclear. Therefore, we examined whether hyperglycemia alters CD4 T cell activation, differentiation and survival. Dendritic cells and T cells were isolated from human peripheral blood and cultured with varying amounts of glucose. Using flow cytometry we found dendritic cells primed in hyperglycemic conditions induced CD4 T cells to exhibit a more activated phenotype capable of mobilization. This was evident by upregulation of the adhesion molecule CD11a. Additionally, these activated CD4 T cells had a lower degree of proliferation and decreased expression of the apoptotic protein caspase-3 compared to CD4 T cells primed by dendritic cells stimulated with a physiological concentration of glucose. We conclude that hyperglycemia drives CD4 T cells towards an activated immunophenotype, which could be assessed via flow cytometry in the clinic to further stratify patients with prediabetes and Type 2 Diabetes and potentially predict those patients at highest risk for progression of the disease.

  • Chemistry
  • Immunology
  • Diabetes Mellitus, Type 2
  • Inflammation
  • Received April 2, 2018.
  • Revision received April 30, 2018.
  • Accepted May 15, 2018.
  • Published by American Society for Clinical Laboratory Science
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American Society for Clinical Laboratory Science: 37 (2)
American Society for Clinical Laboratory Science
Vol. 37, Issue 2
1 Apr 2024
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The Role of Hyperglycemia in CD4 T Cell Survival and Differentiation
Naomi Iwai, Catherine Steib, Amanda Marzo, Nadine M Lerret
American Society for Clinical Laboratory Science May 2018, ascls.118.000331; DOI: 10.29074/ascls.118.000331

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The Role of Hyperglycemia in CD4 T Cell Survival and Differentiation
Naomi Iwai, Catherine Steib, Amanda Marzo, Nadine M Lerret
American Society for Clinical Laboratory Science May 2018, ascls.118.000331; DOI: 10.29074/ascls.118.000331
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Keywords

  • Chemistry
  • Immunology
  • Diabetes Mellitus, Type 2
  • Inflammation

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