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Figures
- Figure 1.
CD4 T cell proliferation after coculture with dendritic cells primed in increasing concentrations of glucose. Peripheral blood–derived CFSE-labeled CD3+ CD4 T cells were cocultured with autologous dendritic cells primed in varying concentrations of glucose. (A) After 7 days in culture, the total CD3+ CD4+ T cells (CD4 T cells) per well were enumerated via flow cytometry. (B) Percentage of CFSE high (top), intermediate (middle), and low (bottom) CD4 T cells was assessed using flow cytometry. CFSE high was defined as 1–2 cellular divisions, CFSE intermediate was defined as 3–4 divisions and CFSE low was defined as >5 cell divisions. (C) Percentage of CD4 T cells expressing caspase-3 was evaluated via flow cytometry. Data represent two independent experiments with 3–6 wells per culture condition. *P = ≦.05
- Figure 2.
Human CD4 T cells increase their CD11a expression when cultured with DCs primed in increasing concentrations of glucose. Peripheral blood–derived human CD4 T cells were cocultured with autologous dendritic cells primed in varying concentrations of glucose. (A) After 7 days in culture, effector status of CD3+ CD4+ T cells was determined by analysis of CD11a expression using flow cytometry. (B) Representative dot plots of this expression and (C) bar graphs are shown. Data represent two independent experiments with 3–6 wells per culture condition. **P = ≦.01; ***P = ≦.0001
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