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Polyoxometalate Induces Susceptibility of Methicillin-Resistant Staphylococcus Aureus to Oxacillin

Katie Wilkinson, Tyler Wilkinson, Candace Adamson, Timothy Herzog, Tracy Covey, Barry Lloyd and Matthew Nicholaou
American Society for Clinical Laboratory Science April 2018, 31 (2) 59-64; DOI: https://doi.org/10.29074/ascls.2018000760

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  • Figure 1.
    Figure 1.

    Coincubation effect of α-K6P2W18O62 · 14H2O on MRSA MIC of MRSA in combination with oxacillin (ug/mL) and 3 different concentrations of α-K6P2W18O62 · 14H2O (uM) prepared by coincubation techniques. Each concentration was run in triplicate and obtained the same MIC value, which are represented by the diamonds. The dashed line represents the oxacillin breakpoint at which Staphylococcus aureus is considered resistant or susceptible.

  • Figure 2.
    Figure 2.

    Toxicity of α-K6P2W18O62 · 14H2O on HCT116 cells. Points represent percentage of cell survival for 5 concentrations of POM (0.5,5, 50 and 500uM). Each concentration was run in duplicate and compared with control samples to calculate the percentage of survival. The dashed line represents a fitted logarithmic curve.

  • Figure 3.
    Figure 3.

    The effects of α-K6P2W18O62 · 14H2O on mecA expression MRSA. Overnight cultures of MRSA were incubated for 3 hours at 37 °C without α-K6P2W18O62 · 14H2O in the presence of 4 µg/mL oxacillin to induce mecA expression [control], α-K6P2W18O62 · 14H2O alone [5uM and 50uM], and combinations of 4 ug/mL oxacillin and α-K6P2W18O62 · 14H2O [Ox + 5uM, Ox + 50uM]. Data represent mean fold changes (error bars = +/- 2 SEM) in mecA expression determined by RT-qPCR. All fold changes values were calculated using a standard curve method using the 16s rRNA gene as the endogenous control and the control sample as the reference.

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Polyoxometalate Induces Susceptibility of Methicillin-Resistant Staphylococcus Aureus to Oxacillin
Katie Wilkinson, Tyler Wilkinson, Candace Adamson, Timothy Herzog, Tracy Covey, Barry Lloyd, Matthew Nicholaou
American Society for Clinical Laboratory Science Apr 2018, 31 (2) 59-64; DOI: 10.29074/ascls.2018000760
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